Open AccessPrevalence of pathogenicity island markers genes in uropathogenic Escherichia coli isolated from patients with urinary tract infectious
Author(s) -
Sima Chahar Tangi,
Elahe Tajbakhsh,
Nazila Arbab Soleimani,
Manochehr Momeni Shahraki
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60909-4
Subject(s) - virulence , pathogenicity island , escherichia coli , pathogenicity , microbiology and biotechnology , biology , urinary system , gene , bacteria , enterobacteriaceae , genetics , endocrinology
Objective: To investigate virulence associated characteristics in uropathogenic Escherichia\udcoli (E. coli) isolates derived from urine specimens, and to investigate the distribution of the\udpathogenicity islands virulence markers (PAIs) among the isolates.\udMethods: In this study, 63 E. coli strains isolated from patients with symptoms of urinary tract\udinfections were collected and all of them were confirmed by biochemical tests and molecular\udtechniques. PCR was conducted by specific pair primers in order to determine the prevalence of\udpathogenicity islands markers.\udResults: Among the 63 examined isolates, PAI IV536 was reported in 53 strains (84.12%)\udshowing the highest prevalence. The distribution of PAI IICFT073, PAI III536, PAI I536, PAI II536, PAI\udJ\ud196, PAI IIJ196, PAI ICFT073 were 41.26%, 4.76%, 34.92%, 20.63%, 1.57%, 31.74% and 72.01%,\udrespectively.\udConclusions: Pathogenicity island markers cause horizontal transfer of genes. Many virulence\udgenes are present in the pathogenicity islands regions in such a way that by deletion and/or\udmutation of these genes, the bacteria would lose its pathogenicity. Knowledge of the molecular\uddetails of uropathogenic E. coli is useful to develop successful strategies for the treatment of\udurinary tract infection and complications associated with urinary tract infections in human