Open AccessA mixture of honey bee products ameliorates the genotoxic side effects of cyclophosphamide
Author(s) -
Maha A. Fahmy,
Neveen A. Hassan,
S. El-Fiky,
Heba Gamal Elalfy
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60904-5
Subject(s) - genotoxicity , cyclophosphamide , sperm , dna fragmentation , bone marrow , andrology , honey bee , chemistry , biology , toxicology , zoology , toxicity , medicine , immunology , botany , biochemistry , apoptosis , genetics , chemotherapy , programmed cell death
Objective: To evaluate the protective role of a mixture of honey bee products (honey, royal jelly\udand pollen grains) against the genotoxicity induced by the anticancer drug cyclophosphamide\ud(CP).\udMethods: The study included chromosomal aberration analysis in mice bone marrow cells,\udinduction of morphological sperm abnormalities, DNA fragmentation and histopathological\udchanges induced in liver cells of mice. CP was injected intraperitoneally at the dose of 20 mg/\udkg body weight. The mixture of honey bee products was administrated orally for different\udperiods of time 5, 10 and 15 days with a dose exactly equivalent to the daily intake of human\udbeings.\udResults: The results revealed that honey mixture ameliorated the genotoxic side effects\udof CP. For chromosomal aberrations the percentage reached 25.20 ± 1.30 for CP treated\udgroup, while it reached half of that value 12.30 ± 0.54 in CP-group pretreated with honey\udmixture for 15 days. Breaks, fragments and multiple aberrations were the most pronounced\udtypes of aberrations induced after CP treatment and honey mixture reduced these types of\udabnormalities. CP induced significant percentage of sperm abnormalities 8.52 ± 0.17 compared\udto control 3.10 ± 0.10. The percentage of sperm abnormalities reached nearly to the control\udvalue in CP- mice treated with honey mixture for 15 days. Honey also reduced the incidence of\udliver DNA damage induced by CP. The results also indicated that CP had a marked damaging\udeffect on liver tissue including severe dilatation, congestion of main blood vessels and massive\udinfiltration of inflammatory cells with irregular general pattern of the tissue. These effects were\udgreatly ameliorated by using oral administration of honey mixture for different periods of time.\udConclusions: The results concluded that honey bee mixture can be used as chemopreventive\udagent for minimizing the genotoxic side effects of the anticancer drug CP and open the field for\udits use in many applications