Open AccessPhenolics composition and antidiabetic property of Brachystegia eurycoma seed flour in high-fat diet, low-dose streptozotocin-induced type 2 diabetes in rats
Author(s) -
Emmanuel Anyachukwu Irondi,
Ganiyu Oboh,
Afolabi Akintunde Akindahunsi,
Aline Augusti Boligon,
Margareth Linde Athayde
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60880-5
Subject(s) - streptozotocin , metformin , chemistry , quercetin , gallic acid , caffeic acid , ellagic acid , kaempferol , chlorogenic acid , catechin , rutin , diabetes mellitus , food science , antioxidant , endocrinology , polyphenol , biochemistry , medicine
Objective: To quantify some major pharmacologically important flavonoids and phenolic acids\udin Brachystegia eurycoma seed flour (BESF) and evaluate its antidiabetic activity in type 2\uddiabetic rats.\udMethod: Flavonoids and phenolic acids were quantified using a reverse-phase high pressure\udliquid chromatrography coupled with diode array detection. Type 2 diabetes was induced in\udrats using high-fat diet, low-dose streptozotocin (HFD/STZ) model, by feeding the rats with\udHFD for 2 weeks followed by single dose administration of STZ (40 mg/kg body weight,\udintraperitoneally). The diabetic rats were later fed BESF-supplemented (10% and 20%) diets,\udor administered with metformin (25 mg/kg b.w.) for 21 days; the control rats were fed basal\uddiet during this period. After the dietary regimen, the rats were sacrificed, and their blood, liver\udand pancreas samples were collected for biochemical assays.\udResults: The flavonoids (catechin, rutin, quercitrin, quercetin and kaempferol) and phenolic\udacids (gallic acid, caffeic, chlorogenic and ellagic acid) were abundant in BESF. BESFsupplemented diets (BESF-SD) significantly (P < 0.05) decreased the levels of fasting blood\udglucose (FBG); ameliorated the lipid profile, and significantly (P < 0.05) increased the hepatic\udglycogen of the diabetic rats. BESF-SD also significantly (P < 0.05) attenuated the oxidative\udstress induced by STZ in the liver and pancreas of the diabetic rats. The ameliorative effects of\ud20% BESF-SD compared (P > 0.05) with metformin administration in some of the biomarkers.\udConclusion: The flavonoids and phenolic acids in BESF may have acted synergistically to\udproduce the observed antidiabetic effects. BESF could therefore be an effective and affordable\uddietary therapy for the management of T2DM; and an excellent source for drug discovery