Open AccessCharacterization of sequence diversity in Plasmodium falciparum SERA5 from Indian isolates
Author(s) -
C.N. Rahul,
K. Shiva Rama Krishna,
Modak Meera,
Sandhya Phadke,
Vidya Rajesh
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60862-3
Subject(s) - haplotype , plasmodium falciparum , biology , genetic diversity , genetics , malaria , allele , sequence analysis , gene , dna sequencing , sequence (biology) , virology , population , immunology , medicine , environmental health
Objective: To characterize the sequence diversity of blood-stage Plasmodium falciparum\udserine repeat antigen-5 (PfSERA5) which is lacking in a malaria-endemic country like India.\udMethods: In this study, parasitic DNA was obtained from field isolates collected from various\udgeographic regions. Subsequently, PfSERA5 gene sequence was PCR amplified and DNA\udsequenced.\udResults: We reported the existence of unique repeat polymorphisms and novel haplotypes\udfor both the octamer repeat (OR) and serine repeat (SR) regions of the N-terminal fragment\udof PfSERA5 from Indian isolates. Several isolates from India were identical to low-frequency\udAfrican haplotypes. Unique finding of our study was an Indian isolate showing deletion in a\udperfectly conserved 14 mer sequence within octamer repeat. Indian haplotypes reported in this\udstudy were found to be distributed into the three earlier classified allelic clusters of FCR3, K1\udand Honduras showcasing broad diversity as compared to worldwide haplotypes.\udConclusions: This study is the first report on genetic diversity of PfSERA5 antigen from India.\udFurther evaluation of these haplotypes by serotyping would provide useful information for\udinvestigating variant-specific immunity and aid in malaria vaccine research