Open AccessSuperoxide dismutase activity in patients of cerebral malaria
Author(s) -
Adil Raza,
Sumit Kumar Varshney,
Haris M. Khan,
Mohammad Bilal Malik,
Abbas Ali Mehdi,
Indu Shukla
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60856-8
Subject(s) - parasitemia , superoxide dismutase , malaria , buffy coat , cerebral malaria , plasmodium falciparum , oxidative stress , medicine , immunology , biology
Objective: To estimate superoxide dismutase (SOD) activity in erythrocytes infected with\udPlasmodium falciparum in the cases of cerebral malaria.\udMethods: The diagnosis of cerebral malaria was made clinically and by Giemsa stained\udperipheral blood smear examination, quantitative buffy coat (QBC) examination and rapid\udantigen detection test (RDT). Parasitemia per micro litre of blood was evaluated by counting\ud200 white blood corpuscles and used to calculate parasite density considering 8000 white\udblood corpuscles per micro litre. SOD activity was estimated by the method given by Joe M.\udMcCord and Irwin Fridovich spectrophotometrically. Statistical analysis was performed by\udusing SPSS software version 17.\udResults: The SOD activity in the cases was found to be (1.06 ± 0.50) nmol/mL and that in the\udcontrols was (3.55 ± 0.07) nmol/mL. The SOD activity in the cases was significantly decreased\ud(P < 0.05) as compared to the controls. The Pearson’s coefficient of correlation between SOD\udactivity and parasitemia was found to be -0.93 showing strong negative relationship.\udConclusions: There is severe oxidative stress in falciparum malaria due to reactive oxygen\udspecies and supplementation of antioxidants may modify the course and outcome of the\uddisease