Open AccessAnalysis of E and NS proteins of dengue serotypes and identification of active binding sites for drug molecule
Author(s) -
Sekar Vijayakumar,
Ramesh Venkatachalam
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60852-0
Subject(s) - dengue virus , dengue fever , serotype , binding site , biology , ligand (biochemistry) , virology , active site , chemistry , plasma protein binding , biochemistry , receptor , enzyme
Objective: To analyze the E and NS proteins of four dengue virus (DENV) serotypes which\udinvolve in the formation of dengue fever and to find out the active binding sites on the surfaces\udof the above proteins to access with the effective drug molecule to counter dengue.\udMethods: ClustalW analysis for phylogram, BLASTP analysis for E and NS proteins of DENV\udserotypes and Swiss-Prot for 3D structure of E and NS proteins were used.\udResults: E proteins were grouped into two clusters, DENV-1, DENV-2 and DENV-3 forming\udone cluster and DENV-4 alone forming a different serotype. On the surfaces of E proteins of\udfour serotypes, five binding sites for ligands were located in different DENV which the fourth\udbinding site was identified as the active ligand binding site in DENV-2, DENV-3 and DENV-4\udserotypes and second binding site in the DENV-1. Among the seven NS proteins, NS-1, NS-3\udand NS-5 were identified as significant molecules involved in dengue disease.\udConclusions: Binding sites, essential for ligand binding are found on all the E and NS proteins\udwhich active binding sites have been identified