Open AccessModulatory activities of Zingiber officinale Roscoe methanol extract on the expression and activity of MMPs and TIMPs on dengue virus infected cells
Author(s) -
Bharti Sharma,
David C. Klinzing,
John Donnie A. Ramos
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60849-0
Subject(s) - vero cell , zingiber officinale , dengue virus , chemistry , virus , matrix metalloproteinase , traditional medicine , virology , biochemistry , biology , medicine
Objective: To evaluate the effect of methanolic extract of Zingiber officinale (ZOM) rhizome\udon the activity and expression profile of matrix metalloproteinase (MMP)-2, MMP-9 and tissue\udinhibitor of metalloproteinase (TIMP)-1, TIMP-2 at the mRNA level in dengue virus infected\udVero cells.\udMethods: Total phenolic content and [6]-gingerol content in ZOM were determined by utilizing\udFolin-Ciocalteu reagent and high performance liquid chromatography. IC50 value of ZOM for\udVero cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide\udassay. Vero cells were infected with dengue virus to induce MMPs production. Modulatory\udeffect of ZOM on the activity and expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 were\uddemonstrated by using gelatin zymography and real time RT-PCR respectively.\udResults: Amount of total phenolics in ZOM in terms of mg gallic acid equivalents/g was\ud(252.89 ± 0.56) and it possessed (137.32 ± 2.47) mg [6]-gingerol content per gram of extract.\udThe IC\ud50 value of ZOM was 221.5 µg/mL for Vero cells. The activities of MMP-2 and to a\udlesser extent MMP-9 were significantly enhanced in the conditioned media collected from\udthe dengue virus infected Vero cells compared to conditioned media from non-infected cells\udand their activities were significantly inhibited by ZOM in dose-dependent manner. ZOM\udsignificantly downregulated the mRNA expression of MMP-2 and MMP-9 and upregulated the\udmRNA expression of TIMP-1 and TIMP-2 in dengue virus infected Vero cells in concentrationdependent manner.\udConclusions: The results of this study suggest that ZOM may be effective in the control of\uddengue-virus-induced permeability through the reduction of activities and expression of\udproteases which degrade the adhesion molecules between cells. This may provide the basis for\uddeveloping new and effective methods in controlling severe dengue complications