Open AccessTLC profiles and antibacterial activity of Glinus oppositifolius L. Aug. DC. (Molluginaceae) leaf and stem extracts against bacterial pathogens
Author(s) -
Juliana Janet R. Martin-Puzon,
Demetrio L. Valle,
Windell L. Rivera
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60838-6
Subject(s) - antibacterial activity , acinetobacter , antimicrobial , microbiology and biotechnology , pseudomonas aeruginosa , acinetobacter baumannii , chemistry , bacteria , minimum inhibitory concentration , staphylococcus aureus , escherichia coli , biology , chromatography , antibiotics , biochemistry , genetics , gene
Objective: To determine the antibacterial activities and the thin-layer chromatography\ud(TLC) fingerprint profiles of leaf and stem extracts of Glinus oppositifolius L. Aug. DC (G.\udoppositifolius).\udMethods: The leaves and stems were extracted using chloroform, ethanol and methanol as\udsolvents. The antibacterial activity of the extracts were evaluated through disc diffusion,\udminimum inhibitory concentration and bactericidal concentration assays against methicillinresistant Staphylococcus aureus, vancomycin-resistant Enterococcus, extended spectrum\udβ-lactamase-producing, carbapenem-resistant Enterobacteriaceae, and metallo-β-lactamaseproducing Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumanii (A.\udbaumanii). The TLC separation was carried out on leaf and stem ethanol extracts in ethyl\udacetate: n-hexane solvent system. Distinct spots were examined under visible light, UV 254 nm,\udUV 366 nm and after spraying with vanillin-sulfuric acid.\udResults: The leaf extracts revealed antibacterial activities, inhibiting the growth of the nonresistant and multidrug-resistant strains of the Gram-negative bacteria Escherichia coli, P.\udaeruginosa and A. baumanii. The TLC fingerprint profiles demonstrated the presence of various\udphytochemicals in leaf and stem extracts. Leaf extracts exhibited more diverse constituents\udcompared to stem extracts, but some constituents were similar in both plant parts.\udConclusions: G. oppositifolius leaf extracts can be used as new, alternative sources of\udantimicrobials against non-resistant and multidrug-resistant strains of the Gram-negative\udbacteria Escherichia coli, P. aeruginosa and A. baumanii. The TLC profiles represent the\udchemical integrity of G. oppositifolius leaf and stem extracts which form an important and\udpowerful tool for standardization, authentication, quality control and determination of bioactive\udcomponents of G. oppositifolius in any formulation and in powder form