Open AccessComparative effects of the crude methanol/methylene chloride extract and fractions of Senecio biafrae (Oliv. & Hiern) J. Moore on some fertility parameters in immature female Wistar rats
Author(s) -
Landry Lienou Lienou,
Bruno Phélix Telefo,
Charlette Nangué,
Bagora Bayala,
Stéphanie Chekem Goka,
Didiane Mefokou Yemele,
Richard Simo Tagne,
Nathalie Jiatsa Donfack,
Gildas Tetaping Mbemya,
Ana Paula Ribeiro Rodrigues
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60805-7
Subject(s) - methylene , uterus , ethyl acetate , medicine , endocrinology , luteinizing hormone , chemistry , ovary , estrous cycle , biology , hormone , chromatography , organic chemistry
Objective: To assay the comparative effect of Senecio biafrae leaves and stems methanol/\udmethylene chloride extract as well as its hexane, n-butanol and ethyl acetate fractions on\udreproductive function of immature female rat.\udMethods: Various doses of the methanol/methylene chloride extract and fractions were orally\udadministered for 20 consecutive days to immature female rats. The parameters of the rats such\udas body, ovarian and uterus weight, uterine, ovarian and serum proteins, ovarian cholesterol as\udwell as follicle-stimulating hormone, luteinizing hormone, estradiol and progesterone levels\udwere recorded.\udResults: Levels of ovarian cholesterol and protein as well as uterine protein were doubled\udprincipally in animals treated with the ethyl acetate fraction not matter what dose was\udadministered. The animals treated with crude extract presented a significant increase (P < 0.05)\udof 108%, 79% and 135% in serum estradiol level at the dose of 8 mg/kg, 32 mg/kg and 64 mg/\udkg respectively.\udConclusions: Results of the present study provide evidence on the inducing effect of the\udmethanol/methylene chloride extract of S. biafrae and its fractions on immature female rat’s\udfertility parameters in serum, ovary and uterus