Open AccessMolecular characterization of Giardia intestinalis assemblage E from goat kids in Bangladesh
Author(s) -
Mohammad Alamgir Hossain,
Sohana Akter Mina,
Lolo Wal Marzan,
Mohammad Asif Imran Khan Emon,
Rasel Das,
Amam Zonaed Siddiki
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60800-8
Subject(s) - breed , giardia , biology , parasite hosting , feces , veterinary medicine , locus (genetics) , nested polymerase chain reaction , gene , genetic diversity , zoonotic disease , zoology , polymerase chain reaction , genetics , microbiology and biotechnology , population , environmental health , medicine , world wide web , computer science , disease , pathology
Objective: To molecularly characterize Giardia in goat kids to elucidate the possible zoonotic\udhazards in Bangladesh and find out the role of Giardia protozoan parasite as a source of human\udinfection.\udMethods: Fecal samples of 100 goat kids were genotyped by nested PCR amplification of\udβ-giardin gene fragment followed by sequencing and analysis.\udResults: The total prevalence of Giardia in goat kids was 3% (3/100) and the infection is more\udwidespread in younger (P = 0.36), Black Bengal breed (P = 0.81) and female goat kids (P =\ud0.58). Further analysis ofβ-giardin gene locus has shown that the gene clustered in assemblage\udE rather than assemblages A and B.\udConclusions: The present study suggests the low zoonotic transmission frequency from the\udgoat kids and and giardiasis has least epidemiological significance to humans. Further study on\udthis field is prerequisite in terms of broad geographical areas, age groups, sex and evaluation of\udzoonotic significance along with genetic diversity in other host species as well