Open AccessSpoligotyping of Mycobacterium tuberculosis isolates from tuberculosis diagnosed patients at Dilla University Referral Hospital and other private clinics, Southern Ethiopia
Author(s) -
Gebremedhin Gebrezgabiher,
Gebremedhin Romha,
Gobena Ameni
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60792-1
Subject(s) - tuberculosis , mycobacterium tuberculosis , typing , medicine , sputum , mycobacterium tuberculosis complex , referral , epidemiology , molecular epidemiology , veterinary medicine , cluster (spacecraft) , transmission (telecommunications) , microbiology and biotechnology , genotype , biology , pathology , genetics , family medicine , gene , computer science , programming language , electrical engineering , engineering
Objective: To assess Mycobacterium tuberculosis (M. tuberculosis) strains exsisting in Gedeo\udzone and the surrounding areas of the Southern Ethiopia using spoligotyping.\udMethods: A cross sectional study was carried out from February, 2012 to June, 2013 and 97 (76\udsputum and 21 fine needle aspirate) samples were taken from tuberculosis diagnosed patients at\udDilla University Referral Hospital and other private clinics. Culturing, region of difference (RD9)\uddeletion typing and spoligotyping techniques were employed to isolate M. tuberculosis strains.\udResults: Growth of mycobacteria was observed in 35.1% (34/97). Speciation of isolates\udshowed that 91.2% (31/34) of the isolates were M. tuberculosis. Further characterization led\udto the identification of 23 different spoligotype patterns of M. tuberculosis of which 61%\udand 39% displayed unique and cluster patterns, respectively. The most dominant shared type\udwas spoligotype international type 53. Of the 23 strains, 12 have not been registered in the\udinternational spoligotyping database (SpolDB4). Seventy one percent of the strains belonged to\udthe Euro-American lineage.\udConclusions: This study revealed the existence of both genetically diverse and clustered M.\udtuberculosis strains from tuberculosis patients in the area, suggesting reactivation of infection\udand recent transmission, respectively. Molecular epidemiology of M. tuberculosis should be\uddone nationwide in order to set appropriate control measures