Open AccessAntimicrobial activities of Alstonia boonei stem bark, a Nigerian traditional medicinal plant
Author(s) -
Chika C. Ogueke,
Joachim Uwaleke,
Clifford I. Owuamanam,
B.N. Okolue
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60766-0
Subject(s) - antimicrobial , minimum inhibitory concentration , traditional medicine , agar diffusion test , salmonella typhi , chemistry , bark (sound) , pseudomonas aeruginosa , minimum bactericidal concentration , staphylococcus aureus , microbiology and biotechnology , food science , bacteria , biology , escherichia coli , biochemistry , medicine , ecology , genetics , gene
Objective: To determine the in vitro antimicrobial activities of various solvent extracts of stem\udbark of Alstonia boonei, a Nigerian traditional medicinal plant against some microorganisms of\udfood and clinical importance.\udMethods: The antimicrobial activities of crude solvent extracts of stem bark were determined\udusing well in agar diffusion method against Escherichia coli (E. coli), Staphylococcus aureus,\udSalmonella typhi, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Kluyveromyces sp.\udThe minimum inhibitory concentration (MIC) and minimum bactericidal concentration were\uddetermined to establish the antimicrobial potential of extracts.\udResults: The antimicrobial results revealed that ethanol extract produced maximum zone of\udinhibition (23.73 mm) against E. coli. All the extracts had no inhibitory activity on Salmonella\udtyphi and Pseudomonas aeruginosa at the lowest concentration tested (3.2 mg/mL). MIC was\uddetermined at various concentrations and the least MIC (5.8 mg/mL) was produced by the crude\udethanol extract on E. coli, while the least minimum bactericidal concentration (20 mg/mL) was\udalso produced by the ethanol extract on E. coli. Ethanol and chloroform extracts had the highest\udand least inhibitory effects on the isolates, respectively.\udConclusions: The present study has revealed that the solvent extracts of Alstonia boonei stem\udbark possess potent antimicrobial activity that can be harnessed. It may also be a new source\udof antimicrobial compounds that could be used to combat drug resistance which has become a\udglobal challenge