Open AccessAntimutagenicity and phytoconstituents of Egyptian Plantago albicans L.
Author(s) -
Sahar S. El Souda,
Reda S. Mohammed,
Mona M. Marzouk,
Maha A. Fahmy,
Zeinab M. Hassan,
Ayman A. Farghaly
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60764-7
Subject(s) - genotoxicity , phytochemical , chemistry , quercetin , kaempferol , traditional medicine , micronucleus test , ethanol , micronucleus , polysaccharide , chromatography , food science , pharmacology , toxicity , biochemistry , biology , antioxidant , medicine , organic chemistry
Objective: To evaluate the safety and the possible antimutagenic effect of the defatted aqueous\udethanol extract and polysaccharide extract of Plantago albicans L. areal parts on the genotoxicity\udof the anticancer drug cyclophosphamide and to investigate the chemical constituents of these\udextracts.\udMethods: Two doses of each extract (10 and 15 mg/kg body weight) were administered orally to\udalbino mice 7 d prior to treatment of cyclophosphamide (20 mg/kg body weight, i.p). Chromosomal\udaberration analysis and micronucleus test were performed. Phytochemical investigation of\uddefatted aqueous ethanol extract was carried out through chromatographic tools, chemical and\udphysical analysis to know the active constituents of these extracts.\udResults: Groups of mice administered with defatted aqueous ethanol extract and polysaccharide\udextract prior to cyclophosphamide treatment showed statistically significant reduction in the\udpercentage of chromosomal aberrations and micronuclei in bone marrow cells in a dosedependent manner. Defatted aqueous ethanol extract gave more effective protection in\udcomparison with polysaccharide extract. Phytochemical investigation of defatted aqueous\udethanol extract allowed isolation of gallic acid, methylgallate, tri, tetra, penta-galloyl-β-\udglucopyranoside, kaempferol-3-O-β-sophro pyranoside, quercetin-3-O-β-glucopyranoside-7-\udO-α-rhamnpyranoside, kaempferol and quercetin, for the first time from this species.\udConclusions: The results of the present work demonstrated that Plantago albicans\udphytoconstituents play a protective role against genotoxicity of the drug cyclophosphamide