Open AccessIn vitro anthelmintic activity of Millettia auriculata leaves and stems
Author(s) -
Sanjoy K. Das,
S. Ganapaty
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60749-0
Subject(s) - anthelmintic , phytochemical , chloroform , albendazole , traditional medicine , chemistry , thin layer chromatography , terpene , potency , chromatography , in vitro , biology , stereochemistry , biochemistry , medicine , ecology
Objective: To study the anthelmintic activity of phytochemically charaterized chloroform extracts\udof Millettia auriculata leaves and stems.\udMethods: Chloroform extracts of leaves and stems were prepared. Phytochemical characterisation\udinvolved preliminary screening, optimisation of solvent system in thin layer chromatography (TLC)\udand UV absorption measurement of each bright yellow (in view of a common physical property of\udflavonoids) TLC fraction for each extract. Anthelmintic activity of each extract was carried out for\uddifferent concentrations (10, 20 and 40 mg/mL) using Pheretima posthuma.\udResults: Phytochemical characterisation of the extracts revealed the presence of steroids/\udtriterpenes, phenolics/flavonoids and carbohydrates with optimum TLC-separation in chloroformhexane, 19:1. All the investigated extracts possessed significant anthelmintic activity (P<0.001) at\uda minimal dose of 10 mg/mL when compared with the corresponding concentration of reference\uddrug albendazole. Leaf extract was found to have more efficacy and potency than that of stem and\udalbendazole.\udConclusions: The results of this study indicated that Millettia auriculata can be used as\udanthelmintic drug. It would be also interesting to find out any novel or existing chemical entities\udshowing anthelmintic activity with mechanism of action