Open AccessLaboratory evolution of the entomopathogenic fungus Beauveria bassiana against Anopheles stephensi larvae (Diptera: Culicidae)
Author(s) -
Rahele Veys-Behbahani,
Mona Sharififard,
Navid DinparastDjadid,
Javad Shamsi,
Mohammad Reza Fakoorziba
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60730-1
Subject(s) - beauveria bassiana , anopheles stephensi , bassiana , larva , biology , entomopathogenic fungus , instar , conidium , veterinary medicine , biological pest control , bioassay , toxicology , botany , aedes aegypti , ecology , medicine
Objective: To examine Beauveria bassiana (B. bassiana) fungus bioassay against the larval stages\udof Anopheles stephensi in Iran.\udMethods: The fungal suspension by the concentrations of 1伊1 09, 5伊1 08, 1 08, 5伊1 07 and 1伊\ud107 conidia per milliliter have been prepared in different volumes (2, 4 and 6 mL) and each\udconcentration were added to containers containing 25 Anopheles larva instars 1 and 2. The\udmortality of the dead larvae with abnormal symptoms was recorded as a result of the fungal\udinfection after 24, 48 and 72 h.\udResults: Comparison between the mean mortality rate of Anopheles stephensi larva at different\udconcentrations of B. bassiana strain Iran 429C at 2, 4 and 6 mL showed that there was no\udsignificant relation of the mean mortality rate of larvae at concentrations of 1伊109 and 5伊108,\udand after 48 h resulted in 100% mortality rate of the larvae populations. In addition, there is no\udsignificant differences in the amounts of lethal times (LT) (LT50 and LT90) as LT90 values calculated\udat a concentration of 5伊108 and in volumes 2, 4 and 6 mL were 1.46, 1.36 and 1.08 d, respectively.\udConclusions: B. bassiana strain Iran 429C in 2 mL of 5伊108 concentration or the concentration of\uda 1伊109 mL per 100 mL of water is recommended as the optimal concentration for the control of\udAnopheles larvae. The development of suitable formulations of entomopathogenic fungi may be a\udpromising prospect in the mosquito control programs