Open AccessConsistency of direct microscopic examination and ELISA in detection of Giardia in stool specimen among children
Author(s) -
Zohreh Torabi,
Ali Niksirat,
Saeideh Mazloomzadeh,
Akefeh Ahmadiafshar
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60715-5
Subject(s) - giardia , feces , kappa , medicine , population , veterinary medicine , significant difference , gastroenterology , constipation , infestation , cohen's kappa , biology , microbiology and biotechnology , mathematics , botany , geometry , environmental health , statistics
Objective: To investigate the consistency of direct microscopic examination and ELISA for\uddetermination of Giadia in stool specimen.\udMethod: Study population consisted of children with any clinical symptoms of Giardia\udinfestation since last two weeks. Fresh stool specimen was collected from each child. The stools\udspecimens were assessed by two methods of direct microscopic examination and ELISA.The\uddegree of agreement between direct stool exam and ELISA was calculated by Cohen's kappa\udcoefficient.\udResults: In this study, 124 children with age range 2-12 years were investigated. A total of 64 (61.7%)\udand 79 (65.7%) of children had Giardia by direct stool exam and ELISA test respectively. There was\udassociation between frequency of constipation and Giardia infection (P=0.036). The Cohen's kappa\udcoefficient calculated for degree of agreement between direct stool exam and ELISA showed\udκ=0.756 (P<0.001).\udConclusions: The frequency of Giardia infection in symptomatic children was high and there\udwas high agreement rate between ELISA and direct stool smear