Open AccessIdentification of specific proteins in colorectal cancer patients with Schistosoma mansoni infection as a possible biomarker for the treatment of this infection
Author(s) -
Eshtiyag Abdalla Abdelkareem,
Tan Gim Cheong,
Abdalla Hassan Sharief,
Boon Huat Lim,
Boon Yin Khoo
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60714-3
Subject(s) - clusterin , colorectal cancer , schistosoma mansoni , biomarker , medicine , schistosomiasis , cancer , proteome , gastroenterology , immunology , biology , bioinformatics , biochemistry , apoptosis , helminths
Objective: To identify the associated proteins or antigens in serum of colorectal cancer patients\udwith and without the chronic Schistosoma mansoni (S. mansoni) infection in Sudan.\udMethods: The study included 93 patients with colorectal cancer and/or S. mansoni infection. A\udclinical database of colorectal cancer patients was documented by pathologist and information\udof S. mansoni infection was collected through stool analysis. Blood samples were collected from\udthe identified patients. Geographical distribution of patients and etiological factors of colorectal\udcancer and S. mansoni infection were also discussed in this study. The proteins extracted from\udserum samples of patients using Tri Reagent were electrophoretically separated on sodium\uddodecyl sulfate polyacrylamide gel electrophoresis. Specific protein band was then subjected to\udmatrix laser desorption ionisation-time assistance-of-flight mass spectrometry analysis.\udResults: Stool analysis documented that 40 patients were colorectal cancer patients infected with\udS. mansoni, 26 patients were diagnosed with colorectal cancer only, and 27 patients were infected\udwith S. mansoni only. Approximately 27% of participating patients with colorectal cancer and S.\udmansoni infection in this study came from Gezira State. A specific protein band was identified\udfrom the serum samples of colorectal cancer patients with S. mansoni infection on the separated\udprotein by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The specific protein\udband was then analyzed by matrix laser desorption ionisation-time assistance-of-flight mass\udspectrometry and identified as a clusterin protein.\udConclusions: The study showed that the colorectal cancer patients infected with S. mansoni\udexpressed clusterin protein, indicating that clusterin protein may be a potential biomarker to aid\udin the treatment of colorectal cancer patients infected with S. mansoni in the future