Open AccessBrucellosis: presence of zoonosis infection 3 500 years ago in North of Iran
Author(s) -
Hossein Samadi Kafil,
Seyed baha Hosseini,
Majid Sohrabi,
Mohammad Asgharzadeh
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60707-6
Subject(s) - brucellosis , zoonosis , taqman , biology , veterinary medicine , livestock , polymerase chain reaction , virology , medicine , ecology , biochemistry , gene
Objective: To investigate the presence of brucellosis in graved human and animal buried in the\udsame graves around 3 500 years ago.\udMethods: Two ancient graves in AH2XX1 trench in Gohar Tepe (Behshahr, North of Iran) in which\udhuman and animal were buried in single grave were selected for this study. After DNA extraction\udfrom human and animal tooth samples, TaqMan real time polymerase chain reaction method was\udutilized to check and measure the presence of brucellae bacteria.\udResults: Brucellosis was found in human with 3.9伊105 copy/0.05 gr and in animal with 6.5伊103\udcopy/0.05 gr in grave one. In grave two, 2.6伊105 copy/0.05 gr in human and 7.7伊103 copy/0.05 gr in\udanimal were found.\udConclusions: This study demonstrated the presence of brucellosis infections in this region\udin 3 500 years ago. Also, it showed that the transmission of brucellosis in Gohar Tepe might be\uddue to the frequent use of milk and meat as the main energy source by the residents. Although\udprevious research claimed that brucellosis was limited to the European continent in the Middle\udBronze Age, the current research showed the presence of brucellosis in Middle East region. An\udadvantage of this study over previous research is the use of real time polymerase chain reaction\udwhich is more rigorous for studying the presence of brucellae in ancient objects compared to\udPaleopathological method