Open AccessPhylogenetic analysis of HIV-1 pol gene: first subgenomic evidence of CRF29-BF among Iranian HIV-1 patients
Author(s) -
Kazem Baesi,
Samaneh Moallemi,
Mehrdad Ravanshad
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60690-3
Subject(s) - phylogenetic tree , human immunodeficiency virus (hiv) , genetic diversity , virology , gene , subgenomic mrna , biology , transmission (telecommunications) , recombinant dna , lentivirus , genetics , medicine , rna , viral disease , population , environmental health , electrical engineering , engineering
Objective: To identify the dominant subtype among the HIV-1 strains circulation in Iran.\udMethods: In this cross sectional study 100 HIV positive patients participated. HIV-1 RNA was\udextracted from plasma. RT nested-PCR was performed and the final products were sequenced\udand phylogenetically analyzed; reference sequences were downloaded from Los Alamos, aligned\udwith Iranian pol sequences in the study and analyzed by neighbor-joining method.\udResults: The results of the phylogenetic analysis showed that HIV-1 subtype CRF-35AD was\udthe dominant subtype among HIV-1 infected patients in Iran; this analysis also suggested a new\udcirculating recombinant form that had not previously been identified in Iran: CRF-29BF.\udConclusions: The impact of HIV diversity on pathogenesis, transmission and clinical\udmanagement have been discussed in different studies; therefore, analyses of HIV genetic diversity\udis required to design effective antiretroviral strategies for different HIV subtypes