Open AccessEvaluation of IL-22 polymorphism in patients with visceral leishmaniasis
Author(s) -
Zohreh Babaloo,
Ahad Bazmani,
Hamid Ahmadi,
Safar Sedighi
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60674-5
Subject(s) - genotype , visceral leishmaniasis , gastroenterology , leishmaniasis , polymerase chain reaction , polymorphism (computer science) , medicine , heterozygote advantage , gene polymorphism , dna extraction , immunology , biology , gene , genetics
Objective: To examine the IL-22 gene polymorphism in visceral leishmaniasis (VL) patients in\udcomparison to healthy people as the control group.\udMethods: The blood samples were collected from 236 people, among them 74 were patients with a\udhistory and clinical symptoms of leishmaniasis and 162 were healthy with no signs of infection and\udall of them were residents in endemic area of VL in East Azerbaijan Province. DNA extraction was\udperformed by using salting out method and amplification refractory mutation system-polymerase\udchain reaction was used for detecting the polymorphism of IL-22 promoter (rs2227473A/G).\udResults: In patients group, 9 (12.16%) individuals showed A/A genotype and 29 (39.18%) showed G/\udG genotype. The heterozygote form (A/G) was 36 (48.64%) among this group. In the control group,\udthe proportions of genotypes were 8.02%, 46.09% and 45.06%, respectively.\udConclusions: According to our results, there was no significant association between VL and\udpolymorphism of IL-22 gene promoter (rs2227473A/G)