Open AccessSequencing and genetic analysis of hemagglutinin gene of H9 avian influenza viruses from Iran (2008 to 2011)
Author(s) -
Masoud Soltanialvar,
Ali Bagherpour,
Hassan Norozian
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60651-4
Subject(s) - gene , phylogenetic tree , biology , influenza a virus subtype h5n1 , open reading frame , virology , genetics , hemagglutinin (influenza) , sequence analysis , virus , peptide sequence
Objective: To survey on molecular characterization of Hemagglutinin( HA) gene in H9N2 viruses\udand determine genetic relationship between Iranian H9N2 viruses and other Asian viruses.\udMethods: Ten H9N2 viruses were isolated from commercial broiler chickens in Iran during 2008-\ud2011 and their HA genes were analyzed by reverse transcription-polymerase chain reaction and\udsequencing. Meanwhile, nucleotide sequences (open reading frame) of the HA genes were used for\udphylogenetic tree construction.\udResults: All the isolates possessed the same amino acid motif -R-S-S-R/G-L- at the cleavage\udsite of HA and possessed H at amino acid position 183, A and T at position 190, S at position 138,\udwhereas the representative strains of the other sublineage (except CK/HK/G1/97-like) of H9N2\udviruses had N, E, and A at these positions respectively. Phylogenetic analysis of HA genes showed\udthat it shared a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of\udhuman H5N1 viruses.\udConclusions: The present results indicated the HA genes of H9N2 influenza viruses circulating\udin Iran were well conserved in the past years and the earliest Iranian isolates may be considered\udto represent such a progenitor