Open AccessAntimicrobial constituents from endophytic fungus Fusarium sp.
Author(s) -
Hidayat Hussain,
KarlHeinz Drogies,
Ahmed AlHarrasi,
Zahid Hassan,
Aamer Ali Shah,
Usman Ali Rana,
Ivan Robert Green,
Siegfried Draeger,
Barbara Schulz,
Karsten Krohn
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60650-2
Subject(s) - fusarium oxysporum , bacillus megaterium , antimicrobial , fusarium , fungus , biology , chemistry , microbiology and biotechnology , botany , bacteria , genetics
Objective: To evaluate the antimicrobial potential of fraction of the fungus Fusarium sp. and\udstudy the tentative identification of their active constituents.\udMethods: Six compounds were purified from an fraction of endophytic fungus Fusarium sp.\udusing column chromatography and their structures have been confirmed based on 1H and 13C\udnuclear magnetic resonance spectra, distortionless enhancement by polarization transfer, 2D\udCOSY, heteronuclear multiple quantum correlation and heteronuclear multiple bond correlation\udexperiments. The six isolated compounds were screened for antimicrobial activity using the agar\udwell diffusion method.\udResults: Phytochemical investigation of endophytic fungus Fusarium sp. lead to the isolation and\udidentification of the following compounds viz., colletorin B, colletochlorin B, LL-Z1272β (llicicolin\udB), 4,5-dihydroascochlorin, ascochlorin, and 4,5-dihydrodechloroascochlorin. Colletorin B and\udcolletochlorin B displayed moderate herbicidal, antifungal and antibacterial activities towards\udChlorella fusca, Ustilago violacea, Fusarium oxysporum, and Bacillus megaterium. On the other\udhand LL-Z1272β (llicicolin B) showed moderate antifungal activity towards Ustilago violacea and\udFusarium oxysporum while 4,5-dihydroascochlorin showed strong antibacterial activity towards\udBacillus megaterium. Furthermore, 4,5-dihydrodechloroascochlorin showed very strong antifungal\udactivity towards Eurotium repens.\udConclusions: Antimicrobial activities demonstrated by five of the six isolated compounds clearly\uddemonstrate that these fungi extracts and active compounds present a great potential for the food,\udcosmetic and pharmaceutical industries