Open AccessChemical composition and antimicrobial effect of the essential oil of Zataria multiflora Boiss endemic in Khorasan-Iran
Author(s) -
Avaei Aida,
Mohamadi Sani Ali,
Mahmoodzadeh Vaziri Behrooz
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60649-6
Subject(s) - essential oil , carvacrol , aspergillus niger , thymol , antimicrobial , bacillus cereus , food science , minimum inhibitory concentration , penicillium digitatum , chemistry , penicillium , fungicide , satureja , aspergillus flavus , biology , microbiology and biotechnology , botany , bacteria , genetics
Objective: To determine the composition and antimicrobial effect of Zataria multiflora Boiss.\udessential oil in "in vitro" condition.\udMethods: The chemical composition of the essential oil obtained by hydro-distillation was\udexamined by GC/MS and the antimicrobial effect was studied on the growth of seven microbial\udspecies including Bacillus cereus, Pseudomonas aeruginosa, Proteus vulgaris, Saccharomyces\udcereviciae, Candida utilis, Penicillium digitatum and Aspergillus niger using micro-dilution\udmethod. The minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal\udconcentration were determined.\udResults: Chemical composition analysis identified a total of 43 compounds in which the main\udcomponents were thymol (42.46%), carvacrol (16.85%), р-cymene (10.62%), γ-terpinene (7.26%) and\udα-pinene (3.00%) representing 80.19% of the total oil. Other separated components accounted for\udless than 19.81% of the oil. Results of antimicrobial analysis showed that Bacillus cereus (MIC=50\udand minimum bactericidal concentration=200 µg/mL) was more resistant than two other bacterial\udspecies. Among the tested yeasts, Saccharomyces cereviciae (MIC=200 and minimum fungicidal\udconcentration=1 600 µg/mL) was more resistant than Candida utilis, while among the fungal\udspecies, growth of Penicillium digitatum and Aspergillus niger inhibited at the same concentration\udof oil.\udConclusions: The results of the present study indicated that Zataria multiflora Boiss. essential\udoil had significant (P<0.05) antimicrobial activity