Open AccessDetection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients
Author(s) -
Abdolmajid Ghasemian,
Shahin Najar Peerayeh,
Bita Bakhshi,
Mohsen Mirzaee
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60643-5
Subject(s) - sccmec , staphylococcus aureus , microbiology and biotechnology , linezolid , biology , antibiotics , gene , vancomycin , chromosome , methicillin resistant staphylococcus aureus , genetics , bacteria
Objective: To detect antibiotic resistance pattern, methicillin resistant Staphylococcus aureus (S.\udaureus), staphylococcal cassete chromosome mec (SCCmec) types and accessory gene regulator (agr)\udgroups among isolates of S. aureus.\udMethods: Of 78 S. aureus isolates, we performed antibiotic susceptibility test and then, and\uddetected mecA gene, SCCmec types and agr specificity group genes by PCR assay.\udResults: All the isolates were susceptible to vancomycin and linezolid. The majority of (94.2%)\udof methicillin resistant S. aureus harbored SCCmec type III. We detected agr group I in 45% and\udgroup II in 34.6% of the isolates. The other strains belonged to agr specificity groups III and IV\ud(3.27% and 22.9%, respectively).\udConclusions: The majority of (45%) S. aureus isolates belonged to agr specificity group I. There\udwas no statistical significant relationship between agr and antibiotic resistance and/or clinical\udsigns