Open AccessDetection and characterization of Escherichia coli O157:H7 from feral pigeon in Qom province, Iran
Author(s) -
Hossein Esameili,
Ali Khanjari,
Fatemeh Gholami
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60637-x
Subject(s) - stx2 , escherichia coli , multiplex polymerase chain reaction , feces , veterinary medicine , biology , serology , polymerase chain reaction , microbiology and biotechnology , gene , medicine , shiga toxin , antibody , immunology , biochemistry
Objective: To detect and characterize Escherichia coli (E. coli) O157:H7 in feral pigeons in Qom\udprovince, Iran.\udMethods: In this survey, 290 cloacal samples were obtained from trapped feral pigeons in Qom\udprovince. Microbiological, biochemical and serological examinations were done to detect the E.\udcoli O157:H7. Isolates were subjected to multiplex polymerase chain reaction for the detection of\udstx1, stx2, eaeA and hlyA genes.\udResults: Four samples (1.38%) were positive for E. coli O157:H7 by using O157 and H7 antisera and\udonly one E. coli O157:H7 strain isolated showed the presence of stx1, stx2, eaeA and hlyA genes.\udConclusions: The results of present survey revealed that feral pigeons in Qom province had\udthe potential to be a reservoir of E. coli O157:H7. The low prevalence of E. coli O157:H7 can be\udattributed to sampling each pigeon just once and fecal culture limits, and true prevalence of the E.\udcoli O157:H7 might be higher than our findings