Open AccessEnzymatic and biochemical changes in common carp, Cyprinus carpio (L.) fingerlings exposed to crude leaf extract of Cannabis sativa (L.)
Author(s) -
B. S. Audu,
Malachy N.O. Ajima,
P. C. Ofojekwu
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60636-8
Subject(s) - cyprinus , common carp , bilirubin , albumin , biology , lactate dehydrogenase , phytochemical , gill , biochemistry , food science , enzyme , fish <actinopterygii> , endocrinology , fishery
Objective: To investigate the effects of different concentrations of leaf extract of Cannabis sativa\ud(C. sativa) on the biochemical changes and level of alterations in the enzymatic activities in\udserum, liver and gill of common carp, Cyprinus carpio fingerlings.\udMethods: The fish specimens were exposed to sublethal concentrations (1.88, 3.75, 7.50, 15.00 and\ud30.00 mg/L) of crude leaf extract of C. sativa over a period of 56 d in a static renewable bioassay.\udThe biomarkers studied were alkaline phosphatase, lactate dehydrogenase and total protein in the\udgill, liver and serum while aspartate aminotransferase, alanine aminotransferase bilirubin, total\udbilirubin, albumin, urea acid and cholesterol were determined in the serum only.\udResults: Statistical significant differences (P<0.05) were found in all the biochemical parameters\udof the gills, liver and serum in all the experimental fish exposed to C. sativa compared with the\udcontrols. Phytochemical analysis of the leaves of C. sativa revealed the presence of alkaloids,\udflavonoids, cardiac glycosides, resins, terpins and steroids.\udConclusions: The investigation revealed that prolonged exposure of Cyprinus carpio fingerlings to\udcrude leaf extract of C. sativa cumulated to stressful conditions in the fish. Further investigation\udshould be conducted on the molecular mechanisms of these parameters on the biochemical and\udenzymatic activities as their mode operations are not well studied in fish