Open AccessEthanol extracts of Cassia grandis and Tabernaemontana cymosa inhibit the in vitro replication of dengue virus serotype 2
Author(s) -
Carolina HernándezCastro,
Fredyc Díaz-Castillo,
Marlén Martínez-Gutierrez
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60635-6
Subject(s) - dengue virus , vero cell , cytopathic effect , cytotoxicity , phytochemical , ec50 , cassia , biology , traditional medicine , mtt assay , virus , in vitro , cell culture , dengue fever , microbiology and biotechnology , virology , chemistry , medicine , biochemistry , alternative medicine , traditional chinese medicine , pathology , genetics
Objective: To determine the antiviral activity of ethanol extracts derived from Cassia grandis\udleaves and Tabernaemontana cymosa bark against two dengue virus (DENV) serotype 2 strains\udDENV-2/NG and DENV-2/1 6681 in two cell lines susceptible to infection, VERO and U937.\udMethods: The cytotoxic concentration 50 (CC50) was assessed using the MTT method, and the\udeffective concentration 50 (EC50) was determined using the technique of inhibiting the production\udof infectious viral particles by the plating method. Further testing of dose-response inhibition\udwas performed, and three experimental approaches were evaluated (pre-, trans- and posttreatment) to determine the effect of the extracts according to the time of administration. Finally,\uda preliminary phytochemical analysis for both extracts was performed.\udResults: The cytotoxicity of the extracts was low (CC50>300 µg/mL), and the U937 cell line was\udmore sensitive to the antiproliferative effect of both extracts. When the virus strain-dependent\udselectivities of the extracts were compared, it was found that both extracts were more selective in\udcultures infected with the DENV-2/NG strain than in those infected with the DENV-2/16681 strain.\udA dose-dependent inhibitory effect of the extracts was not observed in any of the evaluations.\udFinally, the highest inhibition was detected with the post-treatment approach with the\udTabernaemontana cymosa extract (99.9% in both cell lines).\udConclusions: A therapy with compounds derived from these extracts would inhibit viral\udreplication and affect steps after viral internalization