Open AccessMosquitocidal efficacy of medicinal plant, Nerium oleander (Apocynaceae), leaf and flower extracts against malaria vector, Anopheles stephensi Liston (Diptera: Culicidae) larvae
Author(s) -
Mohammad Reza Fakoorziba,
Mohammad Djaefar MoemenbellahFard,
Kourosh Azizi,
Fariba Mokhtari
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60623-x
Subject(s) - anopheles stephensi , petroleum ether , larvicide , bioassay , traditional medicine , chloroform , apocynaceae , larva , biology , potency , aedes aegypti , toxicology , botany , chemistry , chromatography , medicine , extraction (chemistry) , in vitro , ecology , biochemistry
Objective: To assess the larvicidal efficacy of five different extracts of Nerium oleander L. (N.\udoleander) against malaria mosquitoes, Anopheles stephensi (An. stephensi), to combat disease.\udMethods: The acetone, benzene, petroleum ether, chloroform, and aqueous extracts from\udleaves and flowers of N. oleander were subjected to distillation process in a Clevenger type glass\udapparatus model soxhlet. Larvicidal bioassays were performed to determine their LC50 and LC90\udvalues, regression equation, and 95% confidence with lower and upper limits were calculated\udusing probit analysis. Mortality effect was recorded after 24 h.\udResults: The data indicated that there were significant differences in the lethality (LC50, LC90)\udvalues of An. stephensi mosquito larvae among most of the various extracts with their controls. The\udorder of potency with five different concentrations of flower extracts was benzene>chloroform>\udacetone>petroleum>water. The order of potency with similarly five different concentrations of leaf\udextracts was chloroform>petroleum>benzene>water>acetone.\udConclusions: It was concluded that there was an overall lethal effect of N. oleander extracts\udagainst mosquito, An. stephensi larvae which could be manipulated to develop a safe and effective\udlarvicide