Open AccessAntimalarial activity of Evolvulus alsinoids Linn.-an in vitro Plasmodium falciparum specific lactate dehydrogenase enzyme inhibition assay
Author(s) -
Neeraj Kumar Sethiya,
Priyadarshan Keluskar,
Sanjay Ingle,
S.H. Mishra
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60612-5
Subject(s) - plasmodium falciparum , enzyme , lactate dehydrogenase , biochemistry , chemistry , methanol , enzyme assay , affinity chromatography , chromatography , biology , malaria , immunology , organic chemistry
Objective: To investigate the effect of standardized methanol extract of Evolvulus alsinoids\udLinn. (E. alsinoids) on Plasmodium falciparum specific lactate dehydrogenase (PfLDH) enzyme\udinhibition.\udMethods: To carry out enzyme inhibition studies, lactate dehydrogenase was cloned from\udPlasmodium falciparum 3D7 strain using expression vector pET28a and expressed in Escherichia\udcoli BL21 (DE3). Protein purification was carried out by Ni-affinity chromatography. This protein\udwas used for the inhibition study. Methanol extract of E. alsinoids was standardized by high\udperformance thin layer chromatography described by previous literature and screened for PfLDH\udenzyme inhibitory activity and compared with gossypol (a known PfLDH enzyme inhibitor).\udResults: It was found that E. alsinoids possesses a compond scopoletin and potentially inhibits\udPfLDH [(25.04依0.51)%].\udConclusions: Methanol extract of E. alsinoids showed significant PfLDH inhibition as evidenced\udfrom the experiments performed. The activity may be attributed to the presence of various\udpolyphenolic and flavanoids compounds