Open AccessComposition of the non-polar extracts and antimicrobial activity of Chorisia insignis HBK. leaves
Author(s) -
Salma Ahmed Mahmoud El Sawi,
Doaa Mahmoud Moawad Mahmoud Hanafy,
Taha Shahat Mohamed Ahmed El Alfy
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60609-5
Subject(s) - petroleum ether , chemistry , ethyl acetate , antimicrobial , bacillus cereus , stigmasterol , ether , chromatography , antibacterial activity , unsaponifiable , organic chemistry , extraction (chemistry) , bacteria , biology , genetics
Objective: To investigate the chemical constituents of the petroleum ether extract and the\udether fraction of the 70% ethanol extract of Chorisia insignis HBK. leaves, as well as screen its\udantimicrobial activity.\udMethods: Different chromatographic methods were applied to investigate the non-polar extracts\udand the diffusion assay method was applied to study the antimicrobial activity.\udResults: A total of 50 compounds from the unsaponifiable matter and 20 fatty acid methyl\udesters were identified from the petroleum ether extract by GC/MS analysis. n-Hentriacontane,\udn-tritriacontane, stigmastanol, 3-methoxy-5, 6-dihydrostigmasterol, 7,8-dihydroergosterol,\ud4-methylcholesterol, cholestanol, multiflorenol, cholest-5-en-3-one, cholest-6-one, 5,6-\uddihydroergosterol, stigmasterol, dihydroalbigenin and 11-methyl-Δ5,7,9,15,17,23-triacont-hex-ene\udwere isolated from the petroleum ether extract. Methyl heptacosanoate and quinic acid ester of\udrhamnose were isolated from the ether fraction of the 70% ethanol extract. Antimicrobial activity\udof the total alcohol extract and the successive fractions showed that the ether and the ethyl\udacetate fractions have potent antibacterial activity against Bacillus subtilis and Bacillus cereus.\udConclusions: The ether and the ethyl acetate fractions could be used in pharmaceutical\udformulations as antibacterial agents against Bacillus subtilis and Bacillus cereus, and further\udclinical trials should be performed in order to support the above investigations