Open AccessXanthine oxidase inhibitory activity of some Leguminosae plants
Author(s) -
Leomel E. Argulla,
Christine L. Chichioco-Hernandez
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60602-2
Subject(s) - cassia , xanthine oxidase , allopurinol , chemistry , traditional medicine , xanthine , cassia tora , biochemistry , enzyme , medicine , alternative medicine , pathology , traditional chinese medicine
Objective: To evaluate the xanthine oxidase inhibitory activity of the methanol leaf extracts\udof following Cassia javanica, Cynometra ramiflora, Cassia fistula, Senna siamea, Tamarindus\udindicus, Intsia bijuga, Cassia spectabilis, Saraca thaipingensis (S. thaipingensis), Caesalpinia\udpulcherrima (C. pulcherrima) and Bauhinia purpurea.\udMethod: The xanthine oxidase inhibitory activity was tested spectrophotometically under\udaerobic conditions. Absorption increments was monitored every 30 seconds for 10 min at 295 nm\udindicating the formation of uric acid. The extracts were also tested for the presence of terpenoids,\udsaponins, tannins, flavonoids, steroids, phenolic compounds, alkaloids and cardiac glycosides.\udResults: All the extracts inhibited the action of xanthine oxidase. S. thaipingensis and C.\udpulcherrima exhibited higher than 50% at a concentration 0.05 mg/mL. The IC50 of S. thaipingensis\udand C. pulcherrima were determined as 0.033 mg/ml and 0.053 mg/mL, respectively while that of\udallopurinol is 0.004 mg/mL.\udConclusion: S. thaipingensis and C. pulcherrima are potentially good sources of xanthine oxidase\udinhibitors