Open AccessPhylogenetic analysis of hepatitis delta virus isolated from HBsAg positive patients in Shahrekord, Iran
Author(s) -
Mahshid Azarbahra,
Elahe Tajbakhsh,
Hassan Momtaz
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60593-4
Subject(s) - hbsag , virology , genotype , phylogenetic tree , sequence analysis , virus , hepatitis b virus , hepatitis delta , sequence (biology) , nucleic acid sequence , biology , gene , genetics
Objective: To find out the phylogenetic background of hepatitis delta virus (HDV) samples\udisolated in Shahrekord, Iran.\udMethods: A total of 350 hepatitis B surface antigen (HBsAg) positive sera samples were found\udfrom blood donors and HBsAg positive patients in blood transfusion center and clinical laboratory\udin Shahrekord, Iran. HDV RNA was extracted using RNXPlus (CinnaGen, Iran). A total of 421 bp\udcorresponding to hepatitis delta antigen have been isolated from HDV in Shahrekord, then were\udamplified in polymerase chain reaction system, sequenced for determining nucleotide sequence\udand compared with identified nucleotide sequences of these genes in other countries.\udResults: Among 350 HBsAg positive samples, we could detect HCV RNA in only two samples. After\udsequencing, the nucleotide sequences had a variability of 1/7-3/0 for HD Ag gene. The greatest\udsequence similarity existed between Iranian HD Ag sequence and JF694493-Iran, U25667-China\udwith a sequence similarity of 99.7% and the least relationship between Iranian HD Ag sequence\udand AF008420-USA with a similarity of 92.9%.\udConclusions: It is suggested that precise genotype of HDV circulating in the region can be\uddetermined by more expansive sampling from different parts of Chahar mahal and Bakhtiari\udprovince and neigh bouring provinces (Esfehan and Khoozestan)