Open AccessFree radical scavenging and antioxidant activity of D-pinitol against 7, 12 dimethylbenz (a) anthracene induced breast cancer in sprague dawley rats
Author(s) -
Thamaraiselvan Rengarajan,
Peramaiyan Rajendran,
Natarajan Nandakumar,
M. Balasubramanian,
Ikuo Nishigaki
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60592-2
Subject(s) - dpph , chemistry , antioxidant , dmba , radical , 7,12 dimethylbenz[a]anthracene , nitric oxide , superoxide , in vivo , superoxide dismutase , biochemistry , organic chemistry , enzyme , biology , microbiology and biotechnology , carcinogenesis , gene
Objective: To investigate whether D-pinitol efficiently scavenge free radicals using various in\udvitro models [1, 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, superoxide anion and total\udantioxidant activity] and in vivo models.\udMethods: Female Sprague-Dawley rats (150-160 g) were divided into four groups and each\udgroup consisting of six animals. Group I and group IV were vector and drug control. The group II\udand group III animals were treated with 7, 12-dimethylbenz(a)anthracene (DMBA) 20 mg/kg body\udweight to induce mammary carcinoma. Rats received cancer bearing Group III animals were\udtreated with D-pinitol at the concentration of 200 mg/kg bodyweight for 45 d orally. Five different\udconcentrations of D-pinitol (100 to 500 µg/mL) were used in in vitro studies.\udResults: The results revealed that D-pinitol efficiently scavenges DPPH radicals and the IC50\udwas found to be 290 µg/mL and it also exhibited a dose dependent antioxidant activity at different\udconcentrations. In addition, the superoxide and nitricoxide radicals were also significantly\udinhibited by D-pinitol at the concentrations of 360 and 390 µg/mL respectively. On the other\udhand, D-pinitol has significantly increased antioxidant enzymes during DMBA induced mammary\udcarcinoma.\udConclusions: It can be concluded from the investigation that D-pinitol has an excellent\udantioxidant activity which could be due to the scavenging capacities on the stable DPPH radicals,\udsuperoxide, nitric oxide and DMBA induced free radicals thereby it exhibits remarkable total\udantioxidant activity