Open AccessAntibacterial activity of crude ethanolic extract and solvent fractions of Ficus pseudopalma Blanco leaves
Author(s) -
Ma. Cristina De Las Llagas,
Librado A. Santiago,
John Donnie A. Ramos
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60589-2
Subject(s) - antibacterial activity , agar diffusion test , bacillus subtilis , chemistry , staphylococcus epidermidis , minimum inhibitory concentration , bacteria , gram positive bacteria , staphylococcus aureus , agar , minimum bactericidal concentration , antimicrobial , agar plate , chloroform , traditional medicine , food science , biology , chromatography , organic chemistry , genetics , medicine
Objective: To investigate the antimicrobial properties of Ficus pseudopalma (F. pseudopalma)\udleaf extracts.\udMethods: The antibacterial properties of F. pseudopalma Blanco crude ethanolic leaf extract,\udand its solvent fractions chloroform (CF), ethylacetate (EF) and water fractions were evaluated\udthrough antibacterial agar disc diffusion method and the minimum inhibitory concentration (MIC)\udwas determined. Five Gram-positive and five Gram-negative bacteria were used for the study.\udResults: The zone of inhibitions obtained from the antibacterial agar diffusion disc method\udshowed that CF, and EF exhibited active (14-19 mm) antibacterial activity against Bacillus subtilis\udUST CMS 1011, and partially active (10-13 mm) antibacterial properties against both Staphylococcus\udaureus ATCC 25923 and Staphylococcus epidermidis ATCC 12228. Water exhibited no antibacterial\udproperties against all microorgranisms tested. The MIC values observed for all Gram-positive\udbacteria tested were >5 mg/mL, except for Bacillus subtilis whose MIC value was 5 mg/mL for CF\udand EF fractions. All extracts exhibited no antibacterial activity against Gram-negative bacteria.\udConclusions: From this study, it can be concluded that F. pseudopalma extracts may be a\udpotential antibacterial agent against Gram-positive bacteria. The antibacterial property may be\udattributed to flavonoids and terpenoids present in the crude ethanolic extract, CF and EF