Open AccessThe 2011 outbreak of dengue virus infection in Malwa region of Punjab, India-an evaluation of various diagnostic tests
Author(s) -
Neerja Jindal,
Renu Bansal,
Nitika Dhuria
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60588-0
Subject(s) - dengue fever , serology , dengue virus , outbreak , antibody , virology , medicine , antigen , immunology
Objective: To compare the efficacy of rapid immunochromatographic test (ICT) and ELISA for\uddetection of dengue specific parameters (NS1 antigen and IgM antibodies) and the association of\udthese parameters with thrombocytopenia.\udMethods: A total of 1 787 serum samples were obtained from the same number of clinically\udsuspected cases of dengue during an outbreak (2011) of dengue in the Malwa region of Punjab.\udAll the samples were subjected to rapid ICT, dengue early ELISA and dengue IgM-capture ELISA\udfor detection of NS1 antigen and IgM antibodies. Platelet counts of all the cases positive for\uddengue infection and 150 cases negative for dengue parameters (control group) were recorded.\udResults: Rapid ICT for NS1 antigen detection showed same specificity (1 00%) but lower\udsensitivity (85.81%) than the dengue early ELISA, while it was 100% and 94.10% respectively for\udIgM antibody detection. The difference in the platelet counts of cases positive and negative for\uddengue specific parameters was stastically insignificant (standard error of proportions=0.017, Z\udvalue=0.84, P value=0.2).\udConclusions: The confirmation of serological diagnosis of dengue should always be based on\udELISA test and ICT and/or thrombocytopenia should not be used as a stand-alone tes