Open AccessExpressions of ASC and caspase-1 but not AIM2 are disrupted in chronic HBV infected patients
Author(s) -
Gholamhossein Hassanshahi,
Seyyed Mohammad Ali Sajadi,
Vahid Mirzaei,
Ali Ravary,
Hossein Khorramdelazad,
Shokofe Moghadam,
Nahid Zianodini,
Vahid Bagheri,
Mohammad Kazemi Arababadi
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60577-6
Subject(s) - aim2 , peripheral blood mononuclear cell , medicine , hepatitis b virus , apoptosis , inflammasome , messenger rna , immunology , real time polymerase chain reaction , virus , virology , gene , biology , inflammation , biochemistry , in vitro
Objective: To determine the mRNA levels of absent in melanoma 2 (AIM2), apoptosis-associated\udspeck like protein containing a caspase recruitment domain (ASC) and caspase-1 in the peripheral\udblood mononuclear cells of chronic hepatitis B virus (CHB) infected patients.\udMethods: This study was conducted on 60 CHB patients and 60 healthy controls and the mRNA\udlevels of AIM2, ASC and caspase-1 were examined in parallel with beta-actin (as housekeeping\udgene) using real-time polymerase chain reaction technique.\udResults: Our results indicated that expression of ASC and caspase-1 but not AIM2 were\udsignificantly decreased in peripheral blood mononuclear cells isolated from CHB patients\udcompared to healthy controls.\udConclusions: According to our results, it is likely that CHB patients are unable to firmly express\udASC and caspase-1 genes and in turn properly activate IL1β and IL-18 subsequent to hepatitis B\udvirus infection. Thus, these results propose a mechanism which almost partially may describe a\udreasonable fact that why the infection is sustained in the CHB patients