Open AccessAssociation of Aeromonas salmonicida subsp. achromogenes in the haemorrhagic blister of cultured carp Cyprinus carpio in West Bengal, India
Author(s) -
Harresh Adikesavalu,
Asit Patra,
Anjan Mondal,
Sandip Banerjee,
Thangapalam Jawahar Abraham
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60498-9
Subject(s) - aeromonas salmonicida , microbiology and biotechnology , cyprinus , biology , fatty acid , antibiotics , antibiotic sensitivity , aeromonas , aeromonas hydrophila , oxytetracycline , ciprofloxacin , bacteria , biochemistry , fishery , fish <actinopterygii> , genetics
Objective: To study the association of Aeromonas salmonicida subsp. achromogenes in the\udhaemorrhagic blister of cultured exotic carp Cyprinus carpio in West Bengal, India, its cellular\udfatty acid composition and antibiotic sensitivity.\udMethods: The phenotypic characterization and antibiotic sensitivity of bacterial flora of diseased\udfish were performed. The whole cellular fatty acid composition of Aeromonas salmonicida (A.\udsalmonicida) was determined by MIS Sherlock automatic identification system.\udResults: Aeromonas hydrophila, A. salmonicida and Pseudomonas alcaligenes were isolated\udfrom the haemorrhagic blister. The fatty acid methyl esters pattern confirmed the A. salmonicida\udstrain as A. salmonicida subsp. achromogenes. Unsaturated fatty acid 16:1 w7c/16:1 w6c (39.09%),\udsaturated fatty acid 16:0 (26.84%) and the mono-unsaturated fatty acids 18:1 w7c (8.89%) and 16:1\udiso I/14:0 3OH (8.49%) were the most common fatty acids, which accounted for 83.31% of the total\udfatty acids. A. salmonicida subsp. achromogenes was highly susceptible to broad spectrum\udantibiotics such as chloramphenicol, ciprofloxacin, co-trimoxazole, gentamycin, nitrofurantoin\udand oxytetracycline.\udConclusions: The finding on the presence of A. salmonicida in carps necessitates molecular level\udstudy on establishing the prevalence of this bacterium in Indian aquaculture systems especially\udon its free-living viable but non-culturable state