Open AccessIn vitro cytotoxic and apoptotic activity of four Persian medicine plants on human leukemia and lymphoma cells
Author(s) -
Fatemeh Zare Shahneh,
Samira Valiyari,
Abbas Azadmehr,
Reza Hajiaghaee,
Ali Bandehagh,
Behzad Baradaran
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60480-1
Subject(s) - raji cell , apoptosis , leukemia , peripheral blood mononuclear cell , cytotoxic t cell , cell culture , u937 cell , cytotoxicity , biology , programmed cell death , lymphoma , microbiology and biotechnology , chemistry , pharmacology , immunology , in vitro , biochemistry , genetics
Objective: To investigate the cytotoxic and apoptotic activity of Ferulago angulata, Echinophora\udplatyloba, Salvia officinalis and Chelidonium majus on leukemia and lymphoma cell lines, nonHodgkin ’s B-cell lymphoma (Raji), human leukemic monocyte lymphoma (U937), human acute\udmyelocytic leukemia (KG-1A) cell lines and peripheral blood mononuclear cells.\udMethods: C ytotoxicity was determined by the 3 - (4, 5 -dimethylthiazol- 2-yl )- 2,\ud5-diphenyltetrazolium bromide assay. Cell viability assay was done using trypan blue exclusion\udexperiments and cell death was identified as apoptosis using death detection ELISA.\udResults: Our results demonstrated that the extracts dose and time dependently suppressed\udthe proliferation of three leukemia and lymphoma tumor cell lines (KG-1A, U937 and Raji) with\udascending order of IC50 values, while peripheral blood mononuclear cells were not significantly\udaffected. Nucleosome productions in apoptotic KG-1A, U937, and Raji cells were significantly\udaugmented in a time-dependent manner and paralleled the anti-proliferative activity of the\udextracts.\udConclusions: The extracts were found to time- and dose-dependently inhibit the proliferation of\udKG-1A, U937, and Raji cells possibly via an apoptosis-dependent pathway