Open AccessCardioprotective effect of hydroalcoholic extract of Tecoma stans flowers against isoproterenol induced myocardial infarction in rats
Author(s) -
I Shanmukha,
Vijay Kumar Merugumolu,
Ramachandra Setty Siddamsetty
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60474-6
Subject(s) - antioxidant , lactate dehydrogenase , lipid peroxidation , superoxide dismutase , traditional medicine , catalase , pharmacology , chemistry , glutathione , medicine , biochemistry , enzyme
Objective: To investigate the cardioprotective effect of 70% ethanolic extract of Tecoma stans (T.\udstans) flowers against isoproterenol-induced myocardial infarction in rat myocardium.\udMethods: Wister rats were pretreated with 70% ethanolic extract of T. stans flowers (250\udand 500 mg/kg) orally for 14 d and then intoxicated with isoproterenol [200 mg/(kg · day), s.c.]\udfor 2 consecutive d. The biochemical markers for myocardial infarction such as alanine\udaminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine kinase, total\udcholesterol, triglycerides, low density lipoproteins and high density lipoproteins were determined.\udIn addition the antioxidant status on heart tissue is also evaluated by testing the activities of\udantioxidant enzymes such as lipid peroxidation, superoxide dismutase, reduced glutathione and\udcatalase.\udResults: The results indicated that pretreatment with 70% ethanolic extract of T. stans flowers\udprevented fall in antioxidants and retarded elevation of cardiac damage markers in isoproterenol\udtreated rats, significantly. In addition, these findings were evidently supported by the remarkable\udprotection revealed in the histopathological studies, even GC-MS analysis data also substantiated\udout investigation.\udConclusions: It was concluded that, in addition to poly phenolics, some of the phyto fragments\udfound during GC-MS analysis might also contributed to the cardiac protection offered by the\udextract