Open AccessAdenovirus-mediated overexpression of gamma interferon in murine bone marrow-derived dendritic cells affects their viability and activity
Author(s) -
Mirza Ali Mofazzal Jahromi,
Seyed Younes Hosseini,
Mahmood Bozorgmehr,
Kazem Baesi,
Kayhan Azadmanesh,
Zuhair Mohammad Hassan,
Seyed Mohammad Moazzeni
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60470-9
Subject(s) - bone marrow , cd86 , viability assay , interferon , interferon gamma , biology , cell culture , virology , immunology , immune system , t cell , genetics
Objective: To explore the effects of induction of Gamma-interferon(IFN-γ) production by\udadenoviral vectors (AdVs) on dendritic cells (DCs) viability and activity.\udMethods: AdVs were propagated in human embryonic kidney 293 cell lines and DCs were derived\udfrom mouse bone marrow using GM-CSF-IL-4-enriched media. The DCs were infected by either\udAdVs-green fluorescent protein or AdVs-IFN-γ during a 48 h culture. Phenotypic and functional\udcharacteristics of AdV-infected DCs were measured by flowcytometry-based methods.\udResults: Our results displayed that AdVs-IFN-γ could significantly induce DCs necrosis.\udFurthermore, AdVs-IFN-γ-infected DCs efficiently expressed the CD86 molecules.\udConclusions: According to our finding, AdV-IFN-γ is able to affect murine bone marrowderived DC viability and activity