Open AccessHistopathology of mammary gland in Staphylococcus aureus induced mastitis in mice
Author(s) -
Jayaraj F Chinchali,
B.B. Kaliwal
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60463-1
Subject(s) - staphylococcus aureus , mastitis , histopathology , mammary gland , inoculation , infiltration (hvac) , histopathological examination , biology , pathogenicity , pathology , pathogenesis , andrology , microbiology and biotechnology , medicine , bacteria , genetics , physics , cancer , breast cancer , thermodynamics
Objective: To investigate the histopathological changes of mouse mammary tissue damage in\uddose and durational pathogenicity of Staphylococcus aureus (S. aureus) inoculums.\udMethods: The minimal tissue inflammation dose of S. aureus inoculum was determined by\udinoculating the lactating mice mammary gland to observe the mortality rate at 24, 48, 72 and 96 h\udof infection for each inoculum dilutions. To study dose pathogenicity, lactating mice were divided\udinto one control and four induced groups of 5 mice each. S. aureus inoculums doses of 0.8伊101, 1.4\ud伊102, 2伊102 and 2.8伊103 colony forming unit were inoculated to the induced groups II, III, IV and\udV respectively. Mice were sacrificed and mammary gland tissues were harvested after 48 h of\udinfection. Similarly to study of durational pathogenicity, a dose of 2伊102 colony forming unit of S.\udaureus inoculums was inoculated to the induced groups II, III, IV and V respectively. Mice were\udsacrificed and mammary gland tissues were harvested after 24, 48, 72 and 96 h of infection. Body\udand mammary gland were weighed and histopathological responses of the tissue damage were\udobserved for both dose and durational pathogenicity ’s of S. aureus inoculums.\udResults: The observations indicated mastitis symptoms, decrease in body and mammary\udgland weights. Histopathological evidences revealed massive infiltration of polymorphonuclear\udneutrophil leukocytes, damage of alveoli and secretory products as the doses and durations of S.\udaureus inoculum increased compared to the control.\udConclusions: The present study revealed that damage in mammary gland tissue of mice\udincreased as the dose and duration of S. aureus inoculums increased