Open AccessIn vitro antiplasmodial activity of marine sponge Clathria vulpina extract against chloroquine sensitive Plasmodium falciparum
Author(s) -
Sundaram Prasanna Kumar,
S. Ravikumar
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60433-3
Subject(s) - sponge , chloroquine , plasmodium falciparum , ic50 , potency , in vitro , traditional medicine , chemistry , incubation , extraction (chemistry) , pharmacology , malaria , biology , chromatography , botany , biochemistry , medicine , immunology
Objective: To explore the antiplasmodial potential of marine sponge Clathria vulpina (C. vulpina)\udagainst chloroquine sensitive Plasmodium falciparum (P. falciparum).\udMethods: The marine sponge C. vulpina was collected from Thondi coast, authenticated and\udsubjected for extraction by soaking in ethanol:water mixture (3:1 ratio). The percentage of extract\udwas calculated. Filter sterilized extracts (100, 50, 25, 12.5, 6.25, 3.125 μg/mL) were screened for\udantiplasmodial activity against chloroquine sensitive P. falciparum. The extract was also tested\udfor its hemolytic activity.\udResults: The percentage yield of extract of C. vulpina was found to be 4.8%. The crude extract\udof C. vulpina showed excellent antiplasmodial activity (IC 50=14.75 μg/mL) which was highly\udcomparable to the positive control chloroquine (IC50=7 μg/mL). Statistical analysis reveals that the\udsignificant antiplasmodial activity (P<0.05) was observed between the concentrations and the time\udof exposure. The chemical injury to erythrocytes was also carried out, which showed that there\udwere no morphological changes in erythrocytes by the ethanolic extracts of sponges after 48 h of\udincubation. The extract showed slight hemolytic activity which almost equal to chloroquine at\ud100 μg/mL concentration (1.023%).\udConclusions: The marine sponge C. vulpina can be used as a putative antiplasmodial drug after\udcompleting successful clinical trials