Open AccessEvaluation of antidiabetic property of Andrographis paniculata powder in high fat and sucrose-induced type-2 diabetic adult male rat
Author(s) -
Anne Williams Augustine,
Akilavalli Narasimhan,
M Vishwanathan,
K. Balasubramanian
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60429-1
Subject(s) - andrographis paniculata , endocrinology , medicine , glucose transporter , insulin , insulin receptor , sucrose , diabetes mellitus , protein kinase b , chemistry , type 2 diabetes , blood lipids , insulin receptor substrate , cholesterol , glucose uptake , pharmacology , insulin resistance , phosphorylation , biochemistry , alternative medicine , pathology
Objective: To evaluate the antidiabetic effect of the aerial part of Andrographis paniculata (A.\udpaniculata) powder (500 mg/kg body weight) in high fat and sucrose-induced type-2 diabetic rat\udmodel.\udMethods: The fasting blood glucose, oral glucose tolerance test, serum insulin, lipid profile,\udmRNA and protein levels of insulin signaling molecules, 14C-2 deoxy glucose uptake and 14C\udglucose oxidation in liver were checked.\udResults: In the type-2 diabetes-induced group, the fasting blood glucose, oral glucose tolerance,\udserum insulin, lipid profile, glucose uptake and oxidation, Akt and glucose transporter 2 mRNA,\udinsulin receptor and glucose transporter 2 protein (both cytosolic and plasma membrane) and\udphosphorylation of insulin receptor substrate 1 and Akt were impaired. A. paniculata was able\udto successfully reinstate this impairment. In addition to this, A. paniculata did not cause a\udhypoglycemic condition in normal rat, affirming its activity in hyperglycemic state alone.\udConclusions: A. paniculata possesses significant antidiabetic and antihyperlipidemic activities\udin high fat and sucrose-induced type-2 diabetic rat and the molecular actions at the level of\udinsulin signaling molecules in liver reinforce it