Open AccessChemical composition and antibacterial activity of Pinus halepensis Miller growing in West Northern of Algeria
Author(s) -
Nadia Fekih,
Hocine Allali,
Salima Merghache,
F. Chaib,
Djamila Merghache,
Mohamed El Amine,
Nassim Djabou,
Alain Muselli,
Boufeldja Tabti,
Jean Costa
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60323-6
Subject(s) - sabinene , essential oil , antimicrobial , chemistry , enterococcus faecalis , myrcene , antibacterial activity , chemical composition , food science , composition (language) , botany , biology , organic chemistry , limonene , bacteria , biochemistry , escherichia coli , genetics , gene , linguistics , philosophy
Objective: To find new bioactive natural products, the chemical composition and to sudy the\udantibacterial activity of essential oil components extracted from the aerial parts of the Algerian\udaromatic plant Pinus halepensis Miller (P. halepensis) (needles, twigs and buds).\udMethods: The essential oil used in this study was isolated by hydrodistillation using a\udClevenger-type apparatus according to the European Pharmacopoeia. The chemical composition\udwas investigated using GC-retention indices (RI) and GC-MS.\udResults: Forty-nine compounds, representing 97.9% of the total collective oil, were identified.\udEssential oil was dominated by hydrocarbon compounds (80.6%) especially monoterpenes (65.5%).\udThe major compounds from ten oils stations were: myrcene (15.2%-32.0%), α-pinene (12.2%-\ud24.5%), E-β-caryophyllene (7.0%-17.1%), terpinolene (1.8%-13.3%), 2-phenyl ethyl isovalerate\ud(4.8%-10.9%), terpinene-4-ol (1.0%-8.2 %) and sabinene (1.5%-6.3%). The intra-species variations\udof the chemical compositions of P. halepensis aerial parts essential oils from ten Algerian sample\udlocations were investigated using statistical analysis. Essential oil samples were clustered in 2\udgroups by hierarchical cluster analysis, according to their chemical composition. The essential\udoil revealed an interesting antimicrobial effect against Lysteria monocytogenes, Enterococcus\udfaecalis, Pseudomonas aeruginosa, Acinetobacter baumanii, Citrobacter freundii and Klebsiella\udpneumoniae.\udConclusions: These results suggest that the essential oil from P. halepensis may be a new\udpotential source as natural antimicrobial applied in pharmaceutical and food industries