Open AccessIncidence of Listeria species in bovine, ovine, caprine, camel and water buffalo milk using cultural method and the PCR assay
Author(s) -
Ebrahim Rahimi,
Hassan Momtaz,
Asma Behzadnia,
Zeinab Torki Baghbadorani
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60313-3
Subject(s) - listeria , raw milk , water buffalo , listeria monocytogenes , herd , food science , biology , camel milk , bovine milk , veterinary medicine , pasteurization , zoology , medicine , bacteria , genetics
Objective: To determine the prevalence rate of Listeria species in bovine, ovine, caprine, camel\udand water buffalo milk in Iran.\udMethods: From September 2010 to December 2011 a total of 260 bulk milk samples including\ud85 bovine, 37 camel, 34 water buffalo, 56 ovine and 48 caprine bulk milk samples were collected\udfrom commercial dairy herds, in Fars and Khuzestan provinces, Iran and were evaluated for the\udpresence of Listeria species using cultural method and the PCR assay.\udResults: Using cultural method, 19 samples (7.3%) were positive for Listeria spp. The highest\udprevalence of Listeria was found in raw water buffalo milk (11.8%), followed by raw bovine milk\ud(10.6%), raw ovine milk (7.1%), and raw caprine milk (4.2%) samples. All 37 camel milk samples\udfrom 20 camel breeding farms were negative for Listeria spp. The overall prevalence of Listeria\udwas 7.3%, in which Listeria innocua was the most recovered species (4.2%); the remaining isolates\udwere Listeria monocytogenes (1.9%), Listeria ivanovii (0.08%) and Listeria seeligari (0.04%). The PCR\udassay could identify 8 Listeria-contaminated milk samples that were negative using the cultural\udmethod.\udConclusions: The results presented in this study indicate the potential risk of infection with\udListeria in people consuming raw and unpasteurized milk