Open AccessAntimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections
Author(s) -
Subramanyam Dasari,
Raju Naidu Devanaboyaina Shouri,
Wudayagiri Rajendra,
Lokanatha Valluru
Publication year - 2014
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(14)60307-8
Subject(s) - antimicrobial , microbiology and biotechnology , lactobacillus , probiotic , vaginal flora , vagina , biology , bacilli , hydrogen peroxide , catalase , bacteria , food science , bacterial vaginosis , fermentation , biochemistry , genetics , antioxidant
Objective: To assess the probiotic nature of Lactobacillus in preventing cervical pathogens by\udstudying the effectiveness of antimicrobial activity against vaginal pathogens.\udMethods: Lactobacilli were isolated from healthy vaginal swabs on selective media and different\udpathogenic bacteria were isolated by using different selective media. The Lactobacillus strains\udwere tested for the production of hydrogen peroxide and antimicrobial compounds along with\udprobiotic properties.\udResults: Of the 10 isolated Lactobacillus strains, strain 1, 3 and 6 are high hydrogen peroxide\udproducers and the rest were low producers. Results of pH and amines tests indicated that\udpH increased with fishy odour in the vaginal fluids of cervicovaginal infection patients when\udcompared with vaginal fluids of healthy persons. The isolates were found to be facultative\udanaerobic, Gram-positive, non-spore-forming, non-capsule forming and catalase-negative\udbacilli. The results of antimicrobial activity of compounds indicated that 280 and 140 µg/mL\udwas the minimum concentration to inhibit the growth of both pathogens and test organisms\udrespectively.\udConclusions: The results demonstrated that Lactobacillus producing antimicrobial compounds\udinhibits the growth of cervical pathogens, revealing that the hypothesis of preventing vaginal\udinfection by administering probiotic organisms has a great appeal to patients, which colonize the\udvagina to help, restore and maintain healthy vagina