Open AccessHPTLC densitometric method for analysis of thymoquinone in Nigella sativa extracts and marketed formulations
Author(s) -
Prawez Alam,
Hasan Soliman Yusufoğlu,
Anwar Alam
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60102-4
Subject(s) - thymoquinone , nigella sativa , chromatography , silica gel , detection limit , chemistry , high performance thin layer chromatography , calibration curve , thin layer chromatography , linear range , ethyl acetate , hexane , traditional medicine , medicine , antioxidant , biochemistry
Objective: To develop a sensitive and accurate high-performance thin layer chromatography\udmethod and to determine the quantity of thymoquinone in in two different N. sativa extracts and\udmarketed formulations.\udMethods: Thymoquinone was seperated on aluminum-backed silica gel 60 F254 plates with\udn-hexane-ethyl acetate 8:2 (%, v/v) as mobile phase.\udResults: A compact band was obtained for thymoquinone at Rf value of 0.48依0.04. The calibration\udplot was linear in the range of 50-700 ng/spot of thymoquinone and the correlation coefficient of\ud0.998 9 was indicative of good linear dependence of peak area on concentration. Limit of detection\ud(8.67 ng/spot), limit of quantification (17.43 ng/spot) accuracy (less than 2) and recovery (ranging\udfrom 98.39-99.17) were found satisfactory.\udConclusions: The developed high-performance thin layer chromatography densitometric method\udwas found cheap, selective, precise and accurate and can be used for routine analysis of N. sativa\udextracts and marketed formulations