Open AccessEfficacy and toxicological evaluation of Coccinia grandis (Cucurbitaceae) extract in male Wistar rats
Author(s) -
Anoja Priyadarshani Attanayake,
Kamani Ayoma Perera Wijewardana Jayatilaka,
Chitra Pathirana,
Lakmini Mudduwa
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60101-2
Subject(s) - toxicity , aqueous extract , cucurbitaceae , body weight , acute toxicity , traditional medicine , alloxan , oral administration , medicine , dose dependence , pharmacology , diabetes mellitus , biology , botany , endocrinology
Objective: To investigate the oral antihyperglycemic effect of aqueous leaf extract of Coccinia\udgrandis (L.) (Cucurbitaceae) along with toxicological effects in alloxan induced diabetic and\udhealthy Wistar rats.\udMethods: A single graded dose of aqueous leaf extract of Coccinia grandis (0.25-2.00 g/kg) was\udadministered orally to alloxan induced (150 mg/kg, i.p. ) diabetic Wistar rats (n=6). In acute toxicity\udassessment, Wistar rats were administered the extract at the same dose range and observed for\udthree days. Sub-chronic toxicity was evaluated by daily administration of the extract for 30 d\udat the dose which showed the optimum antihyperglycemic effect. Signs of toxicity, body weight\udof animals, consumption of food and water were monitored during the study period. The effects\udof the extract on biochemical (lipid parameters and activities of liver enzymes), hematological\udparameters (full blood count) and histopathological effects in organs were assessed at the end of\udthe study.\udResults: The optimum effective dose on glucose tolerance for Coccinia grandis leaf extract\udwas found to be 0.75 g/kg in diabetic rats. The extract neither produced significant changes\udin consumption of food, intake of water, relative weight of organs nor affected biochemical,\udhematological and histopathological parameters (P<0.05).\udConclusions: The extract at a dose of 0.75 g/kg was found to be toxicologically safe as a potential\udantihyperglycemic agent in Wistar rats