Open AccessAntibacterial and antioxidant activities of Vaccinium corymbosum L. leaf extract
Author(s) -
Mehnaz Pervin,
Abul Hasnat,
Beong Ou Lim
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60099-7
Subject(s) - vaccinium , dpph , abts , chemistry , food science , antibacterial activity , antioxidant , lipid peroxidation , enterococcus faecalis , botany , traditional medicine , bacteria , biochemistry , biology , escherichia coli , medicine , genetics , gene
Objective: To investigate antibacterial and antioxidant activity of the leaf extract of tropical\udmedicinal herb and food plant Vaccinium corymbosum L. (V. corymbosum) .\udMethods: Free radical scavenging activity on DPPH, ABTS, and nitrites were used to analyse\udphenolic and flavonoid contents of leaf extract. Other focuses included the determination of\udantioxidant enzymatic activity (SOD, CAT and GPx), metal chelating activity, reduction power, lipid\udperoxidation inhibition and the prevention of oxidative DNA damage. Antibacterial activity was\uddetermined by using disc diffusion method against seven strains of bacteria.\udResults: Results found that V. corymbosum leaf extract had significant antibacterial activity. The\udtested extract displayed the highest activity (about 23.18 mm inhibition zone) against Salmonella\udtyphymurium and the lowest antibacterial activity was observed against Enterococcus faecalis\ud(about 14.08 mm inhibition zone) at 10 mg/ disc. The IC 50 values for DPPH, ABTS and radical\udscavenging activity were 0.120, 0.049 and 1.160 mg/mL, respectively. V. corymbosum leaf extract\udalso showed dose dependent reduction power, lipid peroxidation, DNA damage prevention and\udsignificant antioxidant enzymatic activity.\udConclusions: These findings demonstrate that leaf extract of V. corymbosum could be used as an\udalternative therapy for antibiotic-resistant bacteria and help prevent various free radical related\uddiseases