Open AccessAnti-acetylcholinesterase and antioxidant activities and HPLC-MS analysis of polyphenol from extracts of Nelsonia canescens (Lam.) Spreng.
Author(s) -
Nabèrè Ouattara,
Roland Nâg-Tiéro Meda,
Adama Hilou,
Samson Guenné,
Kiessoun Konaté,
Abou Coulibaly,
Martin Kiendrébéogo,
Jeanne Millogo,
Odile Germaine Nacoulma
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60088-2
Subject(s) - chemistry , caffeic acid , polyphenol , antioxidant , ferulic acid , apigenin , chlorogenic acid , abts , quercetin , trolox , lipid peroxidation , gentisic acid , ethyl acetate , biochemistry , trolox equivalent antioxidant capacity , acetylcholinesterase , traditional medicine , food science , dpph , flavonoid , enzyme , salicylic acid , medicine
Objective: To investigate the anti-acetylcholinesterase and antioxidant activities and to evaluate\udthe major polyphenolic compounds of Nelsonia canescens extracts.\udMethods: The anti-acetylcholinesterase activity was assessed using a kinetic inhibition standard\udmethod. Two methods, ABTS and lipid peroxidation, were used to estimate the antioxidant\udcapacity. Polyphenols profile of the plant extract has been determined with a HPLC-MS method.\udResults: The results showed that butanol extract exhibited the best anti-acetylcholinesterase\udactivity with inhibition percentage of (55.62依1.49)%. The best 3 ethylbenzothiazoline-6-sulphonate\udradical cation scavenging capacity was found for ethyl acetate extract with a value of (56.20\ud依0.77) mg equivalent trolox/g while the crude extract showed the highest inhibition of the rat\udliver lipid peroxidation (52.57依1.20)%. Polyphenols profile revealed the presence of five phenol\udacids (p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid and gentisic acid) and three\udflavonoids (apigenin, luteolin, quercetin).\udConclusions: All the extracts of Nelsonia canescens exhibited antioxidant and AChE inhibition\udcapacities. The active compounds identified and quantified in this species are mainly responsible\udfor these in vitro biological activities and allow to justify its widely use in Burkina Faso traditional\udmedicine